Alpha Lipoic Acid Ameliorates the Artificial Sugar Induced Injury to the Female Reproductive Organs of Mice

• Hira Ashfaq

  Department of Zoology, Govt. Girls Degree College, Pishin, Balochistan, Pakistan

  Author

• Asma Rashid Khan

  Department of Zoology, Govt. Graduate College for Women, Township, Lahore, Punjab, Pakistan.

  Author

• Muddasir Hassan Abbasi

  Department of Zoology, University of Okara, Okara-56300, Punjab, Pakistan

  Author

  https://orcid.org/0000-0001-7856-7013

• Asif Mahmood Qureshi

  School of Zoology, Minhaj University Lahore, Pakistan

  Author

• Mohammad Waqar

  School of Zoology, Minhaj University Lahore, Pakistan

  Author

• Kainat Bilal

  School of Zoology, Minhaj University Lahore, Pakistan

  Author

• Khansa Bajwa

  School of Zoology, Minhaj University Lahore, Pakistan

  Author

Background: High Fructose Corn syrup (HFCS) is one of the most common artificial sweeteners widely used in many foodstuffs. Alpha lipoic acid (ALA) is a natural antioxidant that reduces the level of Reactive Oxygen Species (ROS). This study was aimed to find out, whether alpha lipoic acid can mitigate the toxic effect of High Fructose Corn syrup (HFCS) on the female reproductive organ.

Materials and Methods: 30 female mice were equally divided into three groups. First group (C) referred as Control was orally administered distilled water using an insulin syringe. Dose group HFCSI was administrated with 25% HFCS, while dose group HFCSII was administrated with 25% HFCS and 35mg/ kg of BW ALA for 30 days. After the stipulated time, mice were sacrificed to remove ovaries for further study.

Results: Histological studies showed different defects in the ovaries of the dose group HFCS I. It included granulosa cells having less intact oocytes, injured primordial follicles, cysts development, degraded oocyte, a reduced count of follicles, and disarrangements of Tunica albuginea and ovarian surface epithelium. While treatment with ALA reduced the ovarian damage by improving the histopathological changes caused by HFCS.

Conclusion: The consumption of HFCS may lead to increased oxidative stress (OS) which may leads to distortion of the ovarian morphology as seen in histological sections. ALA is found to have meliorative effects and improved the reproductive pathology, normal count of follicles, and histopathological score by reducing the oxidative stress.

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